Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis

作     者：Xiangdong Xue Yuanyuan Zhao Xu Zhang Chunqiu Zhang Anil Kumar Xiaoning Zhang Guozhang Zou Paul C.Wang Jinchao Zhang Xing-Jie Liang 

作者机构：CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety National Center for Nanoscience and Technology Laboratory of Pharmaceutics School of Medicine Tsinghua University Fu Jen Catholic University College of Chemistry & Environmental Science Chemical Biology Key Laboratory of Hebei Province Hebei University 

出 版 物：《Biophysics Reports》 (生物物理学报(英文))

年 卷 期：2015年第1卷第2期

页      面：61-70页

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 081704[工学-应用化学] 07[理学] 070205[理学-凝聚态物理] 08[工学] 0817[工学-化学工程与技术] 080501[工学-材料物理与化学] 070302[理学-分析化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 083203[工学-农产品加工及贮藏工程] 0702[理学-物理学] 

基　　金：financially supported in part by grants from the State High-Tech Development Plan (2012AA020804 and SS2014AA020708) National Natural Science Foundation of China project (Nos. 30970784, 31170873 and 81171455) National Key Basic Research Program of China (2009CB930200) National Distinguished Young Scholars grant (31225009) from the National Natural Science Foundation of China and Chinese Academy of Sciences (CAS) ‘‘Hundred Talents Program’’ (07165111ZX) support by the ‘‘Strategic Priority Research Program’’ of the Chinese Academy of Sciences, Grant No. XDA09030301 the external cooperation program of BIC, CAS, Grant No. 121D11KYSB20130006 supported in part by NIH/NIMHHD G12MD007597 and Do D W81XWH-10-1-0767 grants the Key Basic Research Special Foundation of Science Technology Ministry of Hebei Province (Grant No. 14961302D) 

主　　题：Glucose MALDI-TOF MS Magnetic nanoparticles N-Glycans Oligosaccharides 

摘      要：In this work,2-(2-aminoethoxy) ethanolblocked phenylboronic acid-functionalized magnetic nanoparticles(blocked PMNPs) were fabricated for selective enrichment of different types of *** phenylboronic acid was designed for capturing the cis-diols moieties on saccharides molecules,and the 2-(2-aminoethoxy) ethanol can deplete the nonspecific absorption of peptides and proteins which always coexisted with *** mass spectrometry analysis,the PMNPs bound saccharides can be directly applied onto the MALDI plate with matrix without removing the *** PMNPs,the simple saccharide(glucose) could be detected in pmol *** complex saccharides can also be reliably purified and analyzed;16 different Nglycans were successfully identified from ovalbumin,and the high-abundance N-glycans can be detected even when the ovalbumin was in very low concentration(2 μg).In human milk,ten different oligosaccharides were identified,and the lactose can still be detected when the human milk concentration was low to 0.01 μL.