Co-Culture of Early Embryo with Human Decidual Stromal Cells in vitro by Improvement of Early Embryo Development

作     者：闫洁 朱桂金 刘建新 艾继辉 YAN Jie, ZHU Guijing, LIU Jianxin , AI Jihui (Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical University, Wuhan 430030)

作者机构：Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical University Wuhan 430030 

出 版 物：《Journal of Huazhong University of Science and Technology(Medical Sciences)》 (华中科技大学学报（医学英德文版）)

年 卷 期：2000年第20卷第1期

页      面：79-81页

核心收录：

学科分类：0710[理学-生物学] 1202[管理学-工商管理] 07[理学] 071008[理学-发育生物学] 

主　　题：human decidual stromal cells embryo co-culture 

摘      要：Summary: An early embryo co-culture system with human decidual stromal cells was established to study its effect on early embryonic cleavage and growth in vitro. Three hundred and eight 2-cell mouse embryos were co-cultured with human decidual stromal cell monolayer in MEM+0. 4 % bovine serum albumin (BSA) and 163 embryos cultured in MEM+15 % FCS alone as control. Among the mouse 2-cell embryos co-cultured with human decidual stromal cells, 72.73 % developed to the morula stage and 67.21 % cavitated to blastocysts with 59. 74 % hatching, as compared with 61. 34 % to morula stage, 48. 47 % to blastocysts and none hatching in the controls, respectively. Co-cultured embryos cleaved slightly faster than controls and showed no or less fragmentation than those in the control. These results suggested that human decidual stromal cells can support early embryonic development and yield a reasonable number of embryos with good quality up to blastocyst stage.