Seasonal variations of tris (2-chloroethyl) phosphate and cytotoxicity of organic extracts in water samples from Wuhan, China

作     者：Youjian Zhang Wenjuan Zhang Jian Hou Xian Wang Wenhong Lu Hongyan Zheng Wei Xiong Junling Liu Jing Yuan 

作者机构：Department of Occupational and Environmental HealthHuazhong University of Science and Technology Key Laboratory of Environment and HealthMinistry of Education & Ministry of Environmental ProtectionState Key Laboratory of Environmental Health (Incubating)School of Public HealthTongji Medical CollegeHuazhong University of Science and Technology Wuhan Centers for Disease Control and Prevention 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2019年第31卷第2期

页      面：299-309页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 

基　　金：supported by the National Natural Science Foundations of China (Nos.81273023 and 81472947) 

主　　题：Tris (2-chloroethyl) phosphate Cytotoxicity Organic extract Apoptosis Necrosis 

摘      要：Tris(2-chloroethyl) phosphate(TCEP) is a typical phosphate flame retardant. Its potential adverse health effects have recently aroused concern. We investigated the seasonal variations of TCEP concentrations in the raw, finished and tap water samples from two drinking water treatment plants(DWTPs) in China, and evaluated the cytotoxicity and apoptosis/necrosis of organic extracts(OEs) in water samples. We enriched TCEP and OEs in water samples by solid-phase extraction method. The TCEP concentrations in water samples were determined by gas chromatography–mass spectrometry. Normal human liver cell line L02 was treated with OEs in the water samples, and then the cytotoxicity and apoptosis/necrosis were measured by 3-(4, 5-dimethyithiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and flow cytometry, respectively. The results showed that cytotoxicities of OEs in raw water samples from both DWTPs in summer and winter were stronger than those in spring and autumn, cytotoxicity of OEs in finished and tap water samples from both DWTPs in summer and autumn were stronger than those in spring and winter. In all seasons, the maximal concentrations(100 mL water/mL cell culture) of OEs in the raw water samples from both DWTPs induced late apoptosis/necrosis. The reasons for seasonal variations of TCEP in water samples and potential toxic effects of other pollutants in the water samples need to be further investigated.