以芳香胺玻璃为载体的固定化的胆固醇脂酶和胆固醇氧化酶

作     者：VoMalik S.Singh 等 V. Malik S. Singh C. S. Pundir * (Biochemistry Research Laboratory, Department of Bio Sciences, M. D. University,Rohtak-124 001 (Haryana), India)

作者机构：BiochemistryResearchLaboratoryDepartmentofBio－SciencesM.D.UniversityRohtak－24001（Haryana）India 

出 版 物：《生物工程学报》 (Chinese Journal of Biotechnology)

年 卷 期：2002年第18卷第2期

页      面：155-161页

核心收录：

学科分类：08[工学] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 

主　　题：胆固醇 胆固醇脂酶 胆固醇氧化酶 固定化 芳香胺玻璃 血清 胆固醇测定 载体 

摘      要：Cholesterol esterase (Cease) from bovine pancreas and cholesterol oxidasc (COD) from Bravibacterium recombinant type have been innobilized individually and co-immobilized onto arylamine glass (pore diameter 55nm) through the process of diazotization. Cease and COD retained 92.65% and 85.54% of the initial activity with conjugation yields of 7.2 mg/g and 8.3mg/g support respectively when imnobilized individually on arylamine glass beads, but retained 89.58% of the initial activity with a conjugation yield of 2.9 mg/g support when co-immobilized on the same support. The effects of pH, temperature, time of incubation, substrate concentration, serum inorganic salts & metabolites, thermal stability, storage stability in cold and reusability on the immobilized enzymes were studied and compared with those of free enzymes. The analytic use of both individually immobilized and co-immobilized enzymes in discrete analysis of total and free cholesterol in serum is demonstrated.