Identification of differentially expressed long non-coding RNAs in human ovarian cancer cells with different metastatic potentials

作     者：Shi-Ping Liu Jia-Xin Yang Dong-Yan Cao Keng Shen 

作者机构：Department of Obstetrics and GynecologyPeking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical Sciences 

出 版 物：《Cancer Biology & Medicine》 (癌症生物学与医学（英文版）)

年 卷 期：2013年第10卷第3期

页      面：138-141页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：this study was supported by an intramural grant from sri ramachandra university  chennai 

主　　题：Neoplasm metastasis ovarian neoplasms RNA, long untranslated 

摘      要：Objective： To identify differentially expressed long non-coding RNAs （lncRNAs） involved in the metastasis of epithelial ovarian cancer. Methods： An in vitro invasion assay was performed to validate the invasive capability of SKOV3 and ***1 cell lines. Total *** was then extracted, and microarray analysis was performed. Moreover, nine lncRNAs were selected for validation using RT-qPCR. Results： Compared with the SKOV3 cells, the ***1 cells significantly improved in the in vitro invasive activity. Of the 4,956 lncRNAs detected in the microarra~ 583 and 578 lncRNAs were upregulated and downregulated, respectivel~ in ***1 cells, compared with the parental SKOV3 cells. Seven of the analyzed lncRNAs （MALAT1, H19, UCA1, CCAT1, LOC645249, LOC100128881, and LOC100292680） confirmed the deregulation found by microarray analysis. Conclusion： LncRNAs clusters were differentially expressed in ovarian cancer cells with varying metastatic potentials. This result indicates that some lncRNAs might exert a partial or key role in epithelial ovarian cancer metastasis. Further studies should be conducted to determine the roles of these lncRNAs in ovarian cancer metastasis.