Assessment of HRP2-Based RDT in Diagnosing Malaria Cases

作     者：Yemou Dieng Doudou Sow Therese Dieng Abdourahmane Sow Babacar Faye Jean Louis Ndiaye Daouda Ndiaye Roger Cl6ment Tine Khadime Sylla Oumar Faye Oumar Ndir Oumar Gaye 

作者机构：Department of Parasitology-Mycology Dakar Universite Cheikh Anta Diop Dakar Fann 5005 Senegal Department of Parasitology-Mycology Dakar Centre HospitaIier Universitaire National de Farm Dakar 5035 Senegal 

出 版 物：《Journal of Life Sciences》 (生命科学（英文版）)

年 卷 期：2012年第6卷第6期

页      面：602-607页

学科分类：090603[农学-临床兽医学] 08[工学] 082204[工学-皮革化学与工程] 09[农学] 0906[农学-兽医学] 0822[工学-轻工技术与工程] 

主　　题：Malaria rapid diagnostic test paediatrics HRP2 antigen cerebrospinal fluid 

摘      要：Background： HRP2-based Rapid Diagnostic Tests （RDTs） for malaria ensure a rational use of artemisinin-based combination therapy （ACT）. The HRP2 antigen can go through the cerebrospinal fluid （CSF）. Purpose： To assess the HRP2-based RDT in malaria and detect the HRP2 in CSF. Methods： From November 2006 to May 2007, all patients under 15 years showing clinical symptoms of malaria were included. RDT was performed on the CSF and peripheral blood. Results： Out of the 951 patients included, 131 （13.7%） were confirmed through RDT and 130 （13.6%） through thick blood smear. Sensitivity and specificity stood at 96.96% and 99.71% respectively, for uncomplicated cases and at 100% and 99.13% for severe cases. Tests to detect HRP2 in the CSF of 52 cases were negative. Conclusion： Tests to detect HRP2 in the CSF could make it possible to diagnose severe malaria cases when peripheral parasitemia would be below the detection threshold.