Analysis on promoter elements of cpc operon from Arthrospira platensis

作     者：LU Yongzhong ZHANG Xuecheng 

作者机构：College of Marine Life Sciences Ocean University of China Qingdao 266003 China Qingdao University of Science and Technology Qingdao 266042 China 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：2008年第27卷第1期

页      面：85-91页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 0908[农学-水产] 07[理学] 0707[理学-海洋科学] 

基　　金：The National Natural Science Foundation of China under contract No.30471317 

主　　题：c-phycocyanin SmartRace transcription promoter cyanobacterium 

摘      要：On the basis of bioinformatics analysis, six putative promoters were isolated from the upstream sequence of c-phycocyanin gene of Arthrospira platensis FACHB341 through site-directed mutagenesis, and the transcriptional pattern of c-phycocyanin gene was determined by SmartRace. Results showed that each isolated promoter drived the expression of gfp in E. coli, and Promoter Ⅲ was the strongest one according to the GFP level. Only one transcript of c-phycocyanin gene was found under experimental conditions, and the transcription start site G was located at the - 285 bp upstream of the start codon, from which it could be inferred that the transcript was from the promotion of Promoter Ⅰ.