Cloning and Identification of A Novel Variant of Human Vascular Endothelial Growth Factor

作     者：过健俐 屈伸 GUO JIANLI;QU SHEN DEPARTMENT OF BIOCHEMISTRY;SCHOOL OF BASIC MEDICAL SCIENCES;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCES AND TECHNOLOGY;WUHAN

作者机构：Department of Biochemistry School of Basic Medical Sciences Tongji Medical College Huazhong University of Sciences and Technology Wuhan 430030 

出 版 物：《Journal of Huazhong University of Science and Technology(Medical Sciences)》 (华中科技大学学报（医学英德文版）)

年 卷 期：2001年第21卷第3期

页      面：191-194页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

主　　题：human vascular endothelia growth factor nested PCR variant molecular clone 

摘      要：A novel variant of human vascular endothelial growth factor (h VEGF165) cDNA was amplified by nested PCR method from the HL60 1 cells and was cloned into a eukaryotic expressing vector pcDNA 3 to construct a recombinant plasmid pCD-h VEGF165. The amplified h VEGF165 cDNA fragment was identified by enzyme digestion and DNA sequencing methods. Also, wild-type hVEGF165 cDNA was obtained, identified and cloned into a eukaryotic expressing vector pcDNA3 by using the same methods. The results of DNA sequencing showed that h VEGF165 cDNA cloned from HL60 1 was 600 bp in size with 8 % of the base sequence in h VEGF165 cDNA being changed as compared with the base sequence in the wild-type hVEGF165 cDNA. The results of sequencing of hVEGF165 which was cloned from HL60 by us were consistent with the reports completely.