CDK1-mediated the onset of apoptosis following ultraviolet radiation

作     者：Xin Wei Leya He Xiaolan Li Hui Xiao Deding Tao Junbo Hu Jianping Gong 

作者机构：Cancer Research Institute/Department of General Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China 

出 版 物：《The Chinese-German Journal of Clinical Oncology》 (中德临床肿瘤学杂志（英文版）)

年 卷 期：2009年第8卷第5期

页      面：279-281页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by grants from the Clinical Key Subject Foundation of Health Ministry of China [Diagnosis and analysis of clinical tumor cell cycle (3)] and Life Science Ministry Director Foundation 

主　　题：CDK1 apoptosis ultraviolet radiation flow cytometry 

摘      要：Objective:To find out whether CDK1 could appear the important role in the cell apoptosis that induced by ultraviolet ***:We constructed two kinds of plasmids, one can expressed CDK1 but mutated the fifth amino acid(group A ) and the other can effectively silence the expression of CDK1(group B).When we transfected these plasmids into the 293 cells and at the same time following ultraviolet radiation, we detected the difference of two group cells apoptosis ratio by subG1 peak assay and want to clarify the mechanism by Western blot ***:Two kinds of plasmids could effectively express in 293 *** apoptosis ratio of group A was higher than the group B(P 0.01).And the apoptosis ratio of group B was lower than the group C(P 0.01).Compared with group A and C, the expression of Bcl2 of group B was significantly ***:CDK1 may take a very important role in the programmed cell death.