Comparative genomics and phylogenetic analysis of S. dysenteriae subgroup

作     者：YANG E BIN Wen PENG Junping ZHANG Xiaobing WANG Jing YANG Jian DONG Jie CHU Yonglie ZHANG Jinghai JIN Qi 

作者机构：School of MedicineXi’an Jiaotong UniversityXi’an 710061China School of Pharmaceutical EngineeringShenyang Pharmaceutical UniversityShenyang 110016China State Key Laboratory for Molecular Virology and Genetic EngineeringBeijing 100176China 

出 版 物：《Science China(Life Sciences)》 (中国科学(生命科学英文版))

年 卷 期：2005年第48卷第4期

页      面：406-413页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：High Technology Research and Development Program of China, (2001AA223011) International Science and Technology Co-operation Project, (2001AA223116) State Key Basic Research Program, (G1999054105) 

主　　题：S.dysenteriae subgroup DNA microarray comparative genomics. 

摘      要：Genomic compositions of representatives of thirteen S. dysenteriae serotypes were investigated by performing comparative genomic hybridization (CGH) with microarray containing the whole genomic ORFs (open reading frames, ORFs) of E. coli K12 strain MG1655 and spe-cific ORFs of S. dysenteriae A1 strain Sd51197. The CGH results indicated the genomes of the serotypes contain 2654 conserved ORFs originating from E. coli. However, 219 intrinsic genes of E. coli including those prophage genes, molecular chaperones, synthesis of specific O antigen and so on were absent. Moreover, some specific genes such as type II secretion system associ-ated components, iron transport related genes and some others as well were acquired through horizontal transfer. According to phylogenic trees based on genetic composition, it was demon-strated that A1, A2, A8, A10 were distinct from the other S. dysenteriae serotypes. Our results in this report may provide new insights into the physiological process, pathogenicity and evolution of S. dysenteriae.