Effects of aspirin on number,activity and inducible nitric oxide synthase of endothelial progenitor cells from peripheral blood

作     者：Tu-gang CHEN Jun-zhu CHEN~2 Xu-dong XIE Department of Cardiovascular Disease,First Affiliated Hospital,Medical School of Zhejiang University.Hangzhou 310003,China 

作者机构：DepartmentofCardiovascularDiseaseFirstAffiliatedHospitalMedicalSchoolofZhejiangUniversityHangzhou310003China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2006年第27卷第4期

页      面：430-436页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Project supported by Science and Technology Foundation of Zhejiang Province(№021107613) Health Science Studying Foundation of Zhejiang Province(№2003A043) 

主　　题：aspirin endothelial progenitor cells cell adhesion cell count cell movement cell division physiologic neovascularization 

摘      要：Aim:To investigate whether aspirin has an influence on endothelial progenitorcells(EPC).Methods:Total mononuclear cells(MNC)were isolated from periph-eral blood by Ficoll density gradient centrifugation,then cells were plated onfibronectin-coated culture *** 7 d of culture,attached cells were stimu-lated with aspirin(to achieve final concentrations of 1,2,5,and 10 retool/L)for 3,6,12,and 24 *** were characterized as adherent cells that were double positivefor 1,1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine low density lipoprotein(DiLDL)uptake and lectin binding by direct fluorescent *** prolifera-tion and migration were assayed using a 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT)assay and a modified Boyden chamber assay,*** EPC adhesion assay was performed by replating the EPC onfibronectin-coated dishes,and then adherent cells were *** vitrovasculogenesis activity was assayed by using an in vitro vasculogenesis *** nitric oxide synthase(iNOS)was assayed by Western ***:Incubation of isolated human MNC with aspirin decreased the number of *** also decreased the proliferative,migratory,adhesive,and in vitrovasculogenesis capacity of EPC,and also their iNOS levels in a concentration-and time-dependent ***:Aspirin decreases(1)the number ofEPC;(2)the proliferative,migratory,adhesive and in vitro vasculogenesis capaci-ties of EPC;and(3)iNOS levels in EPC.