Cardiac Electrophysiological Differences Between Kunming and C57BL6/J Mice

作     者：Teng Wang Mu Qin He Huang Hong-liang Li Cong-xin Huang 

作者机构：Department of CardiologyRenmin Hospital of Wuhan UniversityCardiovascular Research Institute of Wuhan UniversityWuhan 430060China 

出 版 物：《Chinese Medical Sciences Journal》 (中国医学科学杂志（英文版）)

年 卷 期：2012年第27卷第2期

页      面：80-87页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 08[工学] 0829[工学-林业工程] 071003[理学-生理学] 

基　　金：Supported by the National Natural Science Foundation of China(81070142) Natural Science Foundation of Hubei Province (2011CDB504) 

主　　题：生理差异 昆明 心脏 C57小鼠 全细胞膜片钳技术 KM小鼠 心肌细胞 小鼠模型 

摘      要：Objective To investigate the alterations of cardiac electrophysiological properties and substantial mechanism and find the stable arrhythmia mouse model in Kunming(KM) and C57BL6/J(C57) mice.Methods Electrocardiogram recordings were used to analyze the QT interval in vivo,and monophasic action potential of right and left ventricular epicardium was recorded to elicit changes of action potential duration(APD) in conventional and programmed electrical stimulation(PES).Transient outward potassium current(I to) was recorded via whole-cell patch-clamp technique in single right and left epicardial myocytes.Results QT interval was prolonged in KM mice relative to C57 mice(62.51±4.47 ms vs.52.59±4.85 ms,P0.05).The APD at 50% repolarization of the left ventricular epicardium(18.60±0.91 ms vs.12.90±0.35 ms),and APDs at 50%(17.31±6.05 ms vs.12.00±3.24 ms) and 70% repolarization(36.13±5.32 ms vs.21.95±8.06 ms) of the right ventricular epicardium in KM mice were significantly prolonged compared with C57 mice,respectively(all P0.05).KM mice were more sensitive to PES-induced ventricular tachycardia(25%,3 of 12 hearts),and especially to Burst-induced ventricular tachycardia(50%,6 of 12 hearts) compared with C57 mice,which were 20%(2 of 10 hearts) and 30%(3 of 10 hearts) respectively.I to densities both in the left and right ventricular epicardial myocytes from KM mice were significantly decreased compared with C57 mice,respectively(all P0.01).Conclusion Our data showed that KM mice with the prolonged QT interval and APD are vulnerabilities to ventricular arrhythmia,which are attributed to lower I to densities in ventricular myocytes obtained from KM mice than that from C57 mice.