Immobilization of penicillin G acylase onto amino-modified silica hydrogel

作     者：Weibing DONG Huining HE Junbo GONG Victor CYANG 

作者机构：Tianjin Key Laboratory for Modern Drug Delivery and High EfficiencySchool of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China 

出 版 物：《Frontiers of Chemical Science and Engineering》 (化学科学与工程前沿（英文版）)

年 卷 期：2010年第4卷第1期

页      面：87-90页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

主　　题：mesoporous materials covalent immobilization penicillin G acylase amino-modified silica gel 

摘      要：Amino-modified silica hydrogel(N-MSHG)was prepared by a simple sol-gel processing via the cocondensation of commercial silica sol with *** G acylase(PGA),a model enzyme,was covalently immobilized onto the N-MSHG and then was used for the enzymatic synthesis of *** samples were characterized by Nitrogen sorption analysis,FT-IR and thermal gravimetric analysis(TGA).The results showed that the amino-modified gel was a mesoporous material with an average pore size of 12.64±0.17 *** immobilization process was efficient and the immobilized enzyme showed high catalytic *** yield of the synthesis of amoxicillin in aqueous media was 38%for 2.5 *** sol-gel preparation is simple and shows prominent potential value in industrial processing.