Effects of deltamethrin on the activation or expression of Nrf2 and on the GCS-HS protein level in primary astrocytes with laser scanning confocal microscope

作     者：Huangyuan Li Nian Shi Yufang Zhong Linlin Liu 

作者机构：Doctor of Medicine Department of Occupational and Environmental Hygiene School of Public Health Fujian Medical University Fuzhou 350004 China Department of Health Toxicology MOE Key lab of environment and health School of Public Health Tongji Medical College HuazJmng Unive.rsity ofScience and Technology Wuhan 430030 China 

出 版 物：《Journal of Nanjing Medical University》 (南京医科大学学报（英文版）)

年 卷 期：2006年第20卷第1期

页      面：1-7页

学科分类：1002[医学-临床医学] 1010[医学-医学技术（可授医学、理学学位）] 100215[医学-康复医学与理疗学] 10[医学] 

基　　金：National Natural Science Foundation (30371225) 

主　　题：deltamethrin NF-E2 related factor 2 gmmm-glutamylcysteine synthetase LSCM astrocyte 

摘      要：Objective： To examine the effects of deltamethrin（DM） and tBHQ on the activation of Nrt2 and expression of GCS-HS in primary astrocytes. Methods： Rat primary astrocytes were treated with DM （10μmol/L） and tBHQ （40μmol/L）, for 1 and 6 h respectively. Results： Analysis using immunofluorescent confocal microscopy revealed that tBHQ treatment led to a dramatic increase in the level of Nrt2. The ratio of cytoplasmic Nrt2 protein to nuclear Nrt2 protein was markedly reduced in astrocytes by either tBHQ or DM treatment. The level of Nrf2 was reduced by combined tBHQ with DM treatment for 1 h, but treatment the ratio of cytoplasmic Nrf2 protein to nuclear Nrf2 protein was increased compared with that of the tBHQ only. The expression of GCS-HS in astrocytes was not altered by DM or tBHQ or both in astrocytes. Conclusion： These findings demonstrated for the first time that Nrf2 was activated by pesticides and antioxidants in astrocytes, implicating a tote of Nrf2 in response to pesticide neurotoxicity ingla.