VGluT1- and GAD-immunoreactive terminals in synaptic contact with PAGimmunopositive neurons in principal sensory trigeminal nucleus of rat
VGluT1- and GAD-immunoreactive terminals in synaptic contact with PAGimmunopositive neurons in principal sensory trigeminal nucleus of rat作者机构：Department of Anatomy and K.K. Leung Brain Research Center the Fourth Military Medical University Xi'an China
出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))
年 卷 期：2007年第28卷第2期
页 面：180-184页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学]
基 金：grants from the National Natural Science Foundation of China(No 30470907)
主 题：trigeminal vesicular glutamate transporter 1 glutamic acid decarboxylase phosphateactivated glutaminase rats
摘 要：Aim:To trace the origin of abundant vesicular glutamate transporter l-like immu-noreactive(VGluT1-LI)axon terminals in the dorsal division of the principal sen-sory trigeminal nucleus(Vpd)and the relationships between VGluT 1-LI,as well asthe glutamic acid decarboxylase(GAD)-LI axon terminals,and phosphate-activated glutaminase(PAG)-LI thalamic projecting neurons in the ***:Following unilateral trigeminal rhizotoMy,triple-immunofluorescence histoche-mistry for VGluT1,GAD and PAG and the immunogold-silver method for VGluTlor GAD,combined with the immunoperoxidase method for PAG were performed,***:After unilateral trigeminal rhizotomy,the density of VGluTl-like immunoreactivity(IR)in the Vpd on the lesion side was reduced compared toits contralateral *** the confocal laser-scanning microscope,theVGluT1-LI or GAD-LI axon terminals were observed to be in close apposition tothe PAG-LI thalamic projecting neuronal profiles,and further electron microscopeimmunocytochemistry confirmed that VGluTl-and GAD-LI axon terminals madeasymmetrical and symmetrical synapses upon the PAG-LI neuronal ***:The present results suggest that the VGluTl-LI axon terminals,whichmainly arise from the primary afferents of the trigeminal ganglion,along with thePAG-LI neuronal profiles,form the key synaptic connection involved in sensorysignaling.
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