Nontargeted identification of peptides and disinfection byproducts in water

作     者：Yanan Tang Ying Xu Feng Li Lindsay Jmaiff Steve E.Hrudey Xing-Fang Li 

作者机构：Division of Analytical and Environmental ToxicologyDepartment of Laboratory Medicine and PathologyUniversity of Alberta Department of Computer ScienceUniversity of Alberta 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2016年第28卷第4期

页      面：259-266页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100402[医学-劳动卫生与环境卫生学] 10[医学] 

基　　金：supported by grants from the Natural Sciences and Engineering Research Council of Canada Alberta Health and Alberta Innovates-Energy and Environment Solutions 

主　　题：Nontargeted detection Water analysis Mass spectrometry Peptide Disinfection byproducts 

摘      要：A broad range of organic compounds are known to exist in drinking water sources and serve as precursors of disinfection byproducts（DBPs）.Epidemiological findings of an association of increased risk of bladder cancer with the consumption of chlorinated water has resulted in health concerns about *** are thought to be an important category of DBP precursors in ***,little is known about the actual presence of peptides and their DBPs in drinking water because of their high sample complexity and low *** address this challenge and identify peptides and non-chlorinated/chlorinated peptide DBPs from large sets of organic compounds in water,we developed a novel high throughput analysis strategy,which integrated multiple solid phase extraction（SPE）,high performance liquid chromatography（HPLC）separation,and non-target identification using precursor ion exclusion（PIE）high resolution mass spectrometry（MS）.After MS analysis,structures of candidate compounds,particularly peptides,were obtained by searching against the Human Metabolome Database（HMDB）.Using this strategy,we successfully detected 625 peptides（out of 17,205 putative compounds）and 617 peptides（out of 13,297）respectively in source and finished water *** source and finished water samples had 501 peptides and amino acids in *** remaining 116 peptides and amino acids were unique to the finished *** a subset of 30 putative compounds for which standards were available,25 were confirmed using HPLC-MS *** analyzing the peptides identified in source and finished water,we successfully confirmed three disinfection reaction pathways that convert peptides into toxic DBPs.