Molecular Clone, Expression, and Prediction of Construction and Function to Key Genes of Interleukin Family of Porcine

作     者：JING Zhi-zhong DOU Yong-xi LUO Qi-hui CHEN Guo-hua MENG Xue-lian ZHENG Ya-dong LUO Xue-nong CAI Xue-peng 

作者机构：Key Laboratory of Veterinary Parasitology of Gansu Province/State Key Laboratory of Veterinary Etiological Biology Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046 P.R. China College of Animal Technology Sichuan Agricultural University Yaan 625014 P.R. China 

出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)

年 卷 期：2007年第6卷第7期

页      面：869-876页

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：Key Science and Technology Special Research Program of Gansu Province, (2GS063-A43-013) National High Technology Research and Development Program of China, (2006AAlOA203) 

主　　题：porcine interleukin gene family analysis of homologies prediction of construction 

摘      要：This research was to clone, express, and analyze the structure and function of major molecules of porcine interleukin family. Genes of porcine interleukin family were cloned by RT-PCR from stimulated porcine PBMC by LPS and PHA, and then expressed in E. coli, and the structure and function of these molecules were predicted by ExPASY. The results showed that genes of IL-4, IL-6, and IL-18 were successfully cloned and expressed. Furthermore, the expression products of recombinant IL-4 and IL-6 both have multiple biological activities. By analyzing these genes with the NCBI/GenBank data, the homologies of the nucleotide acid sequence are 99.25, 99.21, and 100%, respectively, and have great species differences when compared with other animal species. The results of the prediction showed that all these molecules contain several phosphorylation, glycosylation, protein kinase, and signal transduction bonding sites in secondary structure, and all are compact globularity protein in space configuration. These characteristics of structure are the basis for their multiple biological functions. The genes, structure and function of key molecular of porcine interleukin family were successfully cloned, expressed, and analyzed in this paper.