Effect of cholecystokinin octapeptide on tumor necrosis factor α transcription and nuclear factor-κB activity induced by lipopolysaccharide in rat pulmonary interstitial macrophages

作     者：Bin Cong Shu-Jin Li Yi-Ling Ling Department of Pathophysiology,Hebei Medical University,Shijiazhuang 050017,Hebei Province,China Yu-Xia Yao Molecular Biological Laboratory,Hebei Medical University,Shijiazhuang 050017,Hebei Province,China Gui-jun Zhu Department of chest surgey,The Fourth Hospital,Hebei Medical University,Shijiazhuang 050017,Hebei Province,China 

作者机构：Bin Cong Shu-Jin Li Yi-Ling Ling Department of Pathophysiology Hebei Medical University Shijiazhuang 050017 Hebei Province China Yu-Xia Yao Molecular Biological Laboratory Hebei Medical University Shijiazhuang 050017 Hebei Province China Gui-Jun Zhu Department of chest surgey The Fourth Hospital Hebei Medical University Shijiazhuang 050017 Hebei Province China 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2002年第8卷第4期

页      面：718-723页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Natural Science Foundation of Hebei Province No.300322 

主　　题：肺间质 巨噬细胞 脂多糖 肿瘤坏死因子-α 核因子-кB 缩肥囊素 八肽 炎症 

摘      要：AIM: To elucidate the anti-inflammatory mechanism ofan intestinal neuropeptide, sulfated cholecystokininoctapeptide (sCCK-8), the effects of sCCK-8 onlipopolysaccharide (LPS)-induced tumor necrosis factorpulmonary interstitial macrophages (PIMs) ***: PIMs from rat were stimulated with LPS(:1mg @ L-1) in the presence or absence of sCCK-8 (10-8-:10-6mol@ L-1) or/and CCK receptor antagonistproglumide (2 mg @ L-1). The expression of TNF-α mRNAwas assayed by reverse transcription polymerase chainreaction (RT-PCR) at 3h of the stimulation, and nuclearelectrophoretic mobility shift assay (EMSA) at 1 h ofat 30 min of the stimulation was detected by ***: sCCK-8, at concentrations from 10-8 mol @ L-1to 10-6 mol @ L-1 obviously inhibited LPS-induced TNF-αdependent manner, P0.05, P0.01. Stimulation PIMsP0.01, which was elevated by sCCK-8, P0.05. Thewere attenuated by CCK receptor antagonistproglumide, P***: sCCK-8 inhibits LPS-induced TNF-α mRNAwhich is mediated through CCK receptors and inhibitinginflammatory mechanisms of sCCK-8.