A Novel Urea Amperometric Biosensor Based on Secretion of Wild Winter Jasmine Petal Cells Modified on Graphite-Epoxy Composite Electrode
A Novel Urea Amperometric Biosensor Based on Secretion of Wild Winter Jasmine Petal Cells Modified on Graphite-Epoxy Composite Electrode作者机构：College of Chemistry and Life Science Shenyang Normal University Shenyang 110034 Liaoning China
出 版 物：《材料科学与工程（中英文B版）》 (Journal of Materials Science and Engineering B)
年 卷 期：2013年第3卷第6期
页 面：369-374页
学科分类：12[管理学] 1204[管理学-公共管理] 082803[工学-农业生物环境与能源工程] 08[工学] 0828[工学-农业工程] 080202[工学-机械电子工程] 0802[工学-机械工程] 120405[管理学-土地资源管理]
主 题：生物传感器 细胞分泌 尿素浓度 复合电极 花瓣 茉莉 环氧树脂 石墨
摘 要：A new kind of amperometric biosensor was designed for detection of urea with a high selectivity, sensitivity and wide detection range based on the secretion of whole living cells of wild winter jasmine petals paste covered on a graphite-epoxy composite basic electrode surface. The petal paste from mashed fresh winter jasmine petals was tightly fixed on the basic electrode surface with teflon thin film to keep it contact with electrode surface. During the mashing process, the winter Jasmine petal cells secrete some species, which are electrochemical active and gives an oxidation peak at 0.316 V (vs. SCE) in differential pulse voltammetric curve. The interactions of secreted species with urea molecules, and results in the decrease the oxidation peak current. The oxidation peak current was decreased linearly with the logarithm of urea concentration in the range of 1.3 ? 10-16~4.57 ? 10-8 M with the detection limit of 1.3 × 10-16 M.
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