Inhibitory Effect of Extracellular Polysaccharide EPS-II from Pseudoalteromonas on Candida adhesion to Cornea in vitro

作     者：CHEN Hao ZHENG Zhou CHEN Peng WU Xiang Gen ZHAO Ge 

作者机构：State Key Lab Cultivation BaseShandong Provincial Key Lab of OphthalmologyShandong Eye InstituteQingdao 266071ShandongChina Key Laboratory of Marine Bio-active SubstancesFirst Institute of OceanographyState Oceanic AdministrationQingdao 266061ShandongChina. 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2012年第25卷第2期

页      面：210-215页

核心收录：

学科分类：090603[农学-临床兽医学] 08[工学] 09[农学] 0906[农学-兽医学] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 

基　　金：partially supported by the National Natural Scientific Foundation (81000369) the Doctor Foundation of Shandong Province (BS2009SW018) 

主　　题：Extracellular polysaccharide EPS-II Candida albicans Cornea Adhesion Inhibitory effect 

摘      要：Objective Fungal keratitis（FK） is a vision-threatening infection,whose treatment requires more effective and safer anti-fungal agent exploitation *** this aim,we focused on the effect of an extracellular polysaccharide on fungal adhesion to human corneal epithelial *** We performed the cytotoxicity assays of the extracellular polysaccharide EPS-II from an antarctic bacterium Pseudoaltermonas and evaluated its inhibitory effect on Candida albicans cells＇ adherence to human corneal epithelial cells（HCECs）.Results EPS-II,which displayed minor cytotoxicity but also promoted proliferation of HCECs,could inhibit the adherence of yeast cells to HCECs in a dose-dependent ***-II could also suppress the subsequent PI3K/AKT signaling pathway,and thereby decrease the expression of early inflammatory *** ExtracellularpolysaccharideEPS-IIwassuggestedasanewnaturalagentforattenuatingFK.