Synthesis of Cell Adhesive Motif RGD Tripeptide by a Novel Chemical Method and Its Purification

作     者：WANG Hua ZHAO Mi-feng MA Cheng-yun JING Wei HUANG Yi-bing HOU Rui-zhen ZENG Hong-bin XU Li ZHANG Xue-zhong 

作者机构：Key Laboratory of Molecular Enzymology and Engineering Jilin University Changchun 130021 P. R. China China-Japan Union Hospital of Jilin University Changchun 130033 P. R. China First Hospital of Jilin University Changchun 130021 P. R. China 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2006年第22卷第5期

页      面：612-616页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100701[医学-药物化学] 10[医学] 

基　　金：Supported by the Special Research Grant from the State Administration of Traditional Chinese Medicine of China( No.2004ZDZX003) 

主　　题：RGD tripeptide Chemical synthesis Purification Arg-Gly-Asp 

摘      要：The cell adhesive motif RGD tripeptide was synthesized by using a novel chemical method. First, Gly-Asp（GD） was synthesized in two steps including the chloroacetylation of free L-aspartic acid and the ammonolysis of the chloroacetylated L-aspartic acid. The yield of chloroacetylated L-aspartic acid was 83.0%. For the ammonolysis of chloroacetylated L-aspartic acid, the yield of the ammonolyzed product was 92. 3%. Second, the coupling between Arg and Gly-Asp was carried out by using the NCA method. The maximum yield of RGD was about 50% at 0℃ and pH = 9. 5. The prepared RGD tripeptide was confirmed by using amino acid component analysis and mass spectrographic analysis.