Effects of estrogen replacement therapy on apoptosis and vascular endothelial growth factor expression in ocular surface epithelial cells:An experimental study

作     者：Fatih zcura Sema Oru Dndar Emel Dikicioglu etin Nahit Beder Mehmet Dndar 

作者机构：Department of OphthalmologyDumlupinar University School of MedicineKutahyaTurkey Department of OphthalmologyAdnan Menderes University School of MedicineAydinTurkey Department of PathologyAdnan Menderes University School of MedicineAydinTurkey Department of UrologyAdnan Menderes University School of MedicineAydinTurkey 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2012年第5卷第1期

页      面：64-68页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the Research Fund of Adnan Menderes University Aydin Turkey 

主　　题：apoptosis conjunctiva cornea estrogen ocular surface vascular endothelial growth factor 

摘      要：AIM:To investigate the effects of estrogen replacement therapy (ERT) on apoptosis and vascular endothelial growth factor (VEGF) expression in ocular surface in an experimental rat model.· METHODS:Forty female,Wistar rats were randomized in 4 groups in the *** ERT (17β-estradiol,10g/kg/day) was administered to the first group without ovariectomy and to the second group with ovariectomy for three *** group had only ovariectomy and fourth group had sham *** rats were sacrificed in estrous cycles determined by vaginal smear test and their right eyes were enucleated at the end of the third *** eyes were analyzed by immunohistochemical method for expressions of caspase-3,bcl-2,VEGF and TUNEL assay.· RESULTS:Caspase-3 expression of conjunctival epithelium was significantly higher in group 3 than group 1 (P =0.005),and group 2 (P =0.007).TUNEL score of conjunctival epithelium was significantly higher in group 3 than group 1 (P =0.006).TUNEL score of corneal epithelium was significantly higher in group 3 than group 2 (P =0.012),and group 4 (P =0.002).There was no significant difference between groups in that bcl-2 and VEGF expressions.· CONCLUSION:We determined increased apoptosis in ocular surface epithelial cells in ovariectomized *** and endogen estrogen decreased the apoptosis,and did not result in difference in VEGF expression between the *** may be beneficial for the treatment of apoptosis-mediated ocular surface disorders such as dry *** studies are needed on this subject for a better understanding of the role of estrogen and to provide a new insight for treatment and prevention of apoptosis-mediated ocular surface disorders.