Separation,Purification and Characterization of Three Endo-polygalacturonases from a Newly Isolated Penicillum oxalicum

作     者：张春晖 李祖明 彭霞薇 贾月 张洪勋 白志辉 ZHANG Chun-hui;LI Zu-ming;PENG Xia-wei;JIA Yue;ZHANG Hong-xun;BAI Zhi-hui

作者机构：Teacher＇s College of Beijing Union University Beijing 100011 China School of Chemical ＆ Environmental Engineering China University of Mining ＆ Technology (Beijing) Beijing 100083 China Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China Graduate University of Chinese Academy of Sciences Beijing 100049 China 

出 版 物：《过程工程学报》 (The Chinese Journal of Process Engineering)

年 卷 期：2009年第9卷第2期

页      面：242-249页

核心收录：

学科分类：08[工学] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 

基　　金：Supported by National Natural Science Foundation of China (No. 30600082) National Key Technology R&D Program of China (No. 2008BADA7B01) Beijing Municipal Commission of Education (No. KM200811417006) 

主　　题：多聚半乳糖醛酸酶 分离 提取 净化 

摘      要：Three endo-polygalacturonases(endoPGs) from a newly isolated Penicillum oxalicum(CGMCC 0907) capable of utilizing waste biomass as growth substrate were separated and purified to homogeneity by ultra-filtration,affinity adsorption chromatography,CM-cellulose column chromatography,and Sephadex G-100 gel filtration chromatography with the overall yield of 64.5% from the crude *** specific activities and recovery rates of endoPG-1,endoPG-2 and endoPG-3 were 1120 U/mg and 21.6%,1350 U/mg and 25.9%,and 1560 U/mg and 17.0%,*** three purified endoPGs had a close molecular weight to 41 kDa as estimated by *** optimum temperature and pH for the function of them were 65℃ and 5.0,55℃ and 5.0,50℃ and 5.5,*** pI and Km values were 5.9 and 0.78 mg/mL,6.0 and 1.2 mg/mL,and 6.1 and 2.0 mg/mL,respectively.