Helicobacter pylori and other Helicobacter species DNA in human bile samples from patients with various hepato-biliary diseases

作     者：Santosh K Tiwari Aleem A Khan Mohd Ibrahim Mohd Aejaz Habeeb C M Habibullah 

作者机构：Center for Liver Research and Diagnostics Deccan College of Medical SciencesKanchanbaghHyderabad 500 058Andhra PradeshIndia Center for Liver Research and Diagnostics Deccan College of Medical SciencesKanchanbaghHyderabad 500 058Andhra PradeshIndia Department of GastroenterologyDeccan College of Medical Sciences Hyderabad 500 058Andhra PradeshIndia 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2006年第12卷第14期

页      面：2181-2186页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by the Department of Biotechnology  Government of India 

主　　题：Helicobacter pylori Bile Hepato-biliary diseases PCR, Sequence analysis 

摘      要：AIM： To investigate the presence of Helicobacter species by nested PCR of 16S rRNA genes followed by the presence of Flelicobacter pylori （H pylori） 16S rRNA, ureA, cagA genes in bile obtained at endoscopic retrograde cholangio-pancreatography （ERCP） from 60 Indian subjects. METHODS： Sixty bile samples were obtained from patients diagnosed with various hepato-biliary diseases and control subjects at ERCP. PCR analysis was carried out using primers for Helicobacter genus 16S rRNA gene and H pylori （16S rRNA, urea and cagA） genes. Gastric H pylori status was also assessed from biopsies obtained at endoscopy from patients with various hepatobiliary diseases and controls. The control group mainly consisted of subjects with gastric disorders. Sequencing analysis was performed to confirm that PCR products with 16S rRNA and cagA primers were derived from H pylori. RESULTS No Helicobacters were grown in culture from the bile samples. Helicobacter DNA was detected in bile of 96.7% and 6.6% of groups Ⅰ and Ⅱ respectively. Ten from group Ⅰ were positive for 16S rRNA and ureA and 9 were positive for cagA gene. In contrast of the 2 from the control, 1 amplified with 16S rRNA, ureA and cagA primers used. The sequences of the 16S rRNA genes and cagA were 99% similar to Helicobacter pylori. CONCLUSION： Helicobacters are associated with the pathogenesis of various hepato-biliary disorders.