Intravascular local gene transfer mediated by protein-coated metallic stent

作     者：袁晋青 高润霖 史瑞文 宋来风 汤健 李永力 唐承君 孟亮 袁卫民 陈在嘉 Yuan JQ;Gao RL;Shi RW;Song LF;Tang J;Li YL;Tang CJ;Meng L;Yuan WM;Chen ZJ

作者机构：中国医学科学院中国协和医科大学阜外心血管病医院冠心病研究室北京100037 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2001年第114卷第10期

页      面：35-37,105页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：ThisstudywassupportedbyagrantfromNationalResearchKeyProjectoftheNinth FiveYearPlan (No 96 90 6 0 2 0 7) 

主　　题：gene transfer  ·  stent   ·  restenosis  ·   adenovirus 

摘      要：Objective To assess the feasibility, efficiency and selectivity of adenovirus-mediated gene transfer to local arterial wall by protein-coated metallic *** A replication-defective recombinant adenovirus carrying the Lac Z reporter gene for nuclearspecific β-galactosidase (Ad-βgal) was used in this study. The coating for metallic stent was made by immersing it in a gelatin solution containing crosslinker. The coated stents were mounted on a 4.0 or 3.0 mm percutaneous transluminal coronary angioplasty (PTCA) balloon and submersed into a high-titer Ad-βgal viral stock (2 × 1010 pfu/ml) for 3 min, and then implanted into the carotid artedes in 4 mini-swines and into the left anterior descending branch of the coronary artery in 2 mini-swines via 8F large lumen guiding catheters. The animals were sacrificed 7 (n=4), 14 (n = 1) and 21 (n = 1 ) days after implantation, respectively. The β-galactosidase expression was assessed by X-gal *** The results showed that the expression of transgene was detected in all animal. In 1 of carotid artery with an intact intima, the β-gal expression was limited to endothelial cells. In vessels with denuded endothelium, gene expression was found in the sub-intima, media and adventitia. The transfection efficiency of medial smooth muscle cells was 38.6%. In 2 animals sacrificed 7 days after transfection, a microscopic examination of X-gal-stained samples did not show evidence of transfection in remote organs and arterial segments adjacent to the treated arterial *** Adenovirus-mediated arterial gene transfer to endothelial, smooth muscle cells and adventitia by protein-coated metallic stent is feasible. The transfection efficiency is higher. The coated stent may act as a good carrier of adenovirus-mediated gene transfer and have a potential to prevent restenosis following PTCA.