TiO_2-functionalized mesoporous materials for sensitive analysis of multi-phosphopeptides

作     者：GUO WeiChao WAN JingJing QIAN Kun YU ChengZhong KONGJiLie YANG PengYuan LIU BaoHong 

作者机构：Department of Chemistry and Institute of Biomedical Sciences Fudan University Shanghai 200433 China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2011年第54卷第8期

页      面：1327-1333页

核心收录：

学科分类：081702[工学-化学工艺] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070205[理学-凝聚态物理] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0702[理学-物理学] 

基　　金：supported by the National Basic Research Program of Chi-na (2007CB714506) the National Natural Science Foundation of China (20735005 & 20925517) Science and Technology Committee of Shanghai (10XD1406000 & 09JC1402600) Shanghai Leading Academic Disci-pline B109 

主　　题：TiO2 mesoporous materials multi-phosphopeptides enrichment 

摘      要：Protein phosphorylation as one of the most important post-translational modifications in mammalian cells regulates numerous biological processes. Here we propose a novel strategy for the selective isolation and sensitive analysis of mul- ti-phosphopeptides based on TiO2-gratfed mesoporous materials, in which MCM-41 and SBA-15 were chosen as the hard templates. The commercialized IMAC and TiO2 nanopartices were further investigated in the phosphopeptide analysis for comparison. The enrichment efficiency was evaluated and measured by MALDI-TOF mass spectrometry. The results indicated that both TiO2-SBA-15 and TiO2-MCM-41 exhibited the preferential affinity to multi-phosphopeptides compared with the other two widely used strategies. The mesoporous TiO2 based protocol showed highly selective and sensitive properties, where phosphopepddes could be identified at femtomole.