Genetic dissection of tetraploid cotton resistant to Verticillium wilt using interspecific chromosome segment introgression lines

作     者：Peng Wang Zhiyuan Ning Ling Lin Hong Chen Hongxian Mei Jun Zhao Bingliang Liu Xin Zhang Wangzhen Guo Tianzhen Zhang 

作者机构：National Key Laboratory of Crop Genetics & Germplasm Enhancement Cotton Research Institute Nanjing Agricultural University Institute of Plant Protection Jiangsu Academy of Agricultural Sciences Cotton Research Institute Xinjiang Academy of Agriculture and Reclamation Sciences 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2014年第2卷第5期

页      面：278-288页

学科分类：0710[理学-生物学] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 090401[农学-植物病理学] 0902[农学-园艺学] 090402[农学-农业昆虫与害虫防治] 

基　　金：supported by the National Natural Science Foundation of China(30730067 and 31171590) the Philosophy Doctoral Fund Program of Xinjiang Bingtuan Group(2010JC01) the Priority Academic Program Development of Jiangsu Higher Education Institutions 

主　　题：Resistance QTL Chromosome Pyramiding Gossypium hirsutum Gossypium barbadense 

摘      要：Verticillium wilt(caused by the pathogen Verticillium dahliae) is of high concern for cotton producers and consumers. The major strategy for controlling this disease is the development of resistant cotton(Gossypium spp.) cultivars. We used interspecific chromosome segment introgression lines(CSILs) to identify quantitative trait loci(QTL) associated with resistance to Verticillium wilt in cotton grown in greenhouse and inoculated with three defoliating V. dahliae isolates. A total of 42 QTL, including 23 with resistance-increasing and 19 with resistancedecreasing, influenced host resistance against the three isolates. These QTL were identified and mapped on 18 chromosomes(chromosomes A1, A3, A4, A5, A7, A8, A9, A12, A13, D1, D2,D3, D4, D5, D7, D8, D11, and D12), with LOD values ranging from 3.00 to 9.29. Among the positive QTL with resistance-increasing effect, 21 conferred resistance to only one V. dahliae isolate, suggesting that resistance to V. dahliae conferred by most QTL is pathogen isolate-specific. The At subgenome of cotton had greater effect on resistance to Verticillium wilt than the Dt subgenome. We conclude that pyramiding different resistant QTL could be used to breed cotton cultivars with broad-spectrum resistance to Verticillium wilt.