Seroepidemiology and genetic characterization of hepatitis E virus in western Yunnan Province

作     者：Li-Juan Li Yuan-Ying Shen Zhi-Qiong Ai Le Guo Cheng-Rui Geng 

作者机构：School of Public HealthDali University 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2014年第7卷第11期

页      面：909-912页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：supported by a grant from the Basic Research General Program of Yunnan Provincial Science and Technology Department(2013FB060) 

主　　题：Hepatitis E virus Phylogenetic analysis Homology Genotype 

摘      要：Objective:To investigate the seroepidemiology and genetic characterization of hepatitis E virus(HEV) in western Yunnan ***:Questionnaire survey was conducted among1638 residents in western Yunnan Province using stratified sampling ***-linked immunosorbent assay was used to detect serum anti-HEV IgG and *** RNA was extracted from patients with serum anti-HEV IgM *** open reading flame 2(ORF2) of HEV that was amplified by nested RT-PCR was sequenced and compared with standard HEV genotypes ***:Serum anti-HEV positive was found in 13.929(228/1638) *** HEV infection rate in males was significantly higher than that in females with a ratio of 1.47(P0.01).20-30 and30-40 years old young men showed the highest incidence.20.57%and 20.78%.***10-20 and 20-30 years old young women exhibited the highest infection rate,11.85%and 15.60%,*** to occupation,the highest HEV infection rate was observed in farmers(20.35%) and migrants(16.50%).We isolated 10 individual HEV isolates from 31 patients with serum anti-HEV IgM *** analysis and phylogenetic analysis indicated that these10 HEV isolates belonged to HEV genotype 4 with the homology of 78.65%-94.71%.Conclusions:The HEV infection rate is high in western Yunnan *** genotype 4 is the leading cause of HEV infection and young farmers and migrants are the main infected population.