Heavy ion and X-ray irradiation alter the cytoskeleton and cytomechanics of cortical neurons

作     者：Yuting Du Jie Zhang Qian Zheng Mingxin Li Yang Liu Baoping Zhang Bin Liu Hong Zhang Guoying Miao 

作者机构：School of Stomatology Lanzhou University School of Nuclear Science and Technology Lanzhou University Department of Radiation Biology and Medicine Institute of Modern Physics Chinese Academy of Sciences School of Civil Engineering and Mechanics Lanzhou University Gansu Provincial Hospital 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2014年第9卷第11期

页      面：1129-1137页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1001[医学-基础医学(可授医学、理学学位)] 08[工学] 1010[医学-医学技术（可授医学、理学学位）] 100106[医学-放射医学] 10[医学] 

主　　题：nerve regeneration radiation brain injury neurons heavy ion X-ray cytoskeleton cytomechanical properties atomic force microscopy neural regeneration 

摘      要：Heavy ion beams with high linear energy transfer exhibit more beneifcial physical and biological performance than conventional X-rays, thus improving the potential of this type of radiotherapy in the treatment of cancer. However, these two radiotherapy modalities both cause inevitable brain injury. The objective of this study was to evaluate the effects of heavy ion and X-ray irra-diation on the cytoskeleton and cytomechanical properties of rat cortical neurons, as well as to determine the potential mechanism of neuronal injury after irradiation. Cortical neurons from 30 new-born mice were irradiated with heavy ion beams at a single dose of 2 Gy and X-rays at a single dose of 4 Gy;subsequent evaluation of their effects were carried out at 24 hours after irradiation. An immunolfuorescence assay showed that after irradiation with both the heavy ion beam and X-rays, the number of primary neurons was signiifcantly decreased, and there was ev-idence of apoptosis. Radiation-induced neuronal injury was more apparent after X-irradiation. Under atomic force microscopy, the neuronal membrane appeared rough and neuronal rigidity had increased. These cell changes were more apparent following exposure to X-rays. Our ifnd-ings indicated that damage caused by heavy ion and X-ray irradiation resulted in the structural distortion and rearrangement of the cytoskeleton, and affected the cytomechanical properties of the cortical neurons. Moreover, this radiation injury to normal neurons was much severer after irradiation with X-rays than after heavy ion beam irradiation.