New simple spectrophotometric method for determination of the binary mixtures(atorvastatin calcium and ezetimibe;candesartan cilexetil and hydrochlorothiazide) in tablets

作     者：Tarek S.Belal Hoda G.Daabees Magdi M.Abdel-Khalek Mohamed S.Mahrous Mona M.Khamis 

作者机构：Pharmaceutical Analytical Chemistry DepartmentFaculty of PharmacyUniversity of AlexandriaElmessalah 21521AlexandriaEgypt Pharmaceutical Chemistry DepartmentFaculty of PharmacyUniversity of AlexandriaElmessalah 21521AlexandriaEgypt 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2013年第3卷第2期

页      面：118-126页

学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学] 

主　　题：Spectrophotometric analysis Ratio spectra Atorvastatin Ezetimibe Candesartan Hydrochlorothiazide 

摘      要：A new simple spectrophotometric method was developed for the determination of binary mixtures without prior *** method is based on the generation of ratio spectra of compound X by using a standard spectrum of compound Y as a *** peak to trough amplitudes between two selected wavelengths in the ratio spectra are proportional to concentration of X without interference from *** method was demonstrated by determination of two drug *** first consists of the two antihyperlipidemics：atorvastatin calcium（ATV） and ezetimibe（EZE）,and the second comprises the antihypertensives：candesartan cilexetil（CAN） and hydrochlorothiazide（HCT）.For mixture 1,ATV was determined using 10 μg/mL EZE as the divisor to generate the ratio spectra,and the peak to trough amplitudes between 231 and 276 nm were plotted against ATV ***,by using 10 μg/mL ATV as divisor,the peak to trough amplitudes between 231 and 276 nm were found proportional to EZE *** curves were linear in the range 2.5-40 mg/mL for both *** mixture 2,divisor concentration was 7.5 μg/mL for both *** was determined using its peak to trough amplitudes at 251 and 277 nm,while HCT was estimated using the amplitudes between 251 and 276 *** measured amplitudes were linearly correlated to concentration in the ranges 2.5-50 and 1-30 μg/mL for CAN and HCT,*** proposed spectrophotometric method was validated and successfully applied for the assay of both drug combinations in several laboratory-prepared mixtures and commercial tablets.