Constructing skin-equivalents using hair follicle stem cells

作     者：Songmei Geng Shengshun Tan 

作者机构：Department of Dermatology the Second Hospital Xi＇an Jiaotong University Xi＇an 710004 China 

出 版 物：《Journal of Nanjing Medical University》 (南京医科大学学报（英文版）)

年 卷 期：2006年第20卷第2期

页      面：97-100页

学科分类：10[医学] 

主　　题：hair follicle stem cell skin-equivalents 

摘      要：Objective： To establish the method of constructing skin-equivalents （SE） using hair follicle stem cells （HFSC）. Methods： K19 positive cells derived from hair were cultivated using serum-free medium KGM and seeded on dermal equivalents （DE）. After the culture between the air-liquid interface for 14 days, SE were harvested and used for evaluation. Results： K19 positive cells chosen as HFSC were located in bulge of out root sheet in hair follicle. Cultivated HFSC could build a fully developed, multi-layered epidermis on the basis of DE, resembling the skin structure. Conclusion： HFSC located in out root sheet can differentiate into kerafinocyte in vitro and be used for SE construction.