STI571 (Glivec) suppresses the expression of vascular endothelial growth factor in the gastrointestinal stromal tumor cell line,GIST-T1

作     者：Toufeng Jin Hajime Nakatani Takahiro Taguchi Takumi Nakano Takehiro Okabayashi Takeki Sugimoto Michiya Kobayashi Keijiro Araki 

作者机构：Department of Tumor Surgery Kochi Medical School Kochi University Nankoku Kochi 783-8505 Japan and Department of General Surgery College of Medical Yanbian University 119 Juzijie Yanji Jilin Province China Department of Tumor Surgery Kochi-Medical School Kochi University Nankoku Kochi 783-8505 Japan Department of Human Health and Medical Science Graduate school of Kuroshio Science Kochi UniversityNankoku Kochi 783-8503 Japan 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2006年第12卷第5期

页      面：703-708页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：内皮生长因子 基因表达 ST1571 胃肠肿瘤 肿瘤血管 

摘      要：AIM:To estimate whether STI571 inhibits the expressionof vascular endothelial growth factor(VEGF)in thegastrointestinal stromal tumor(GIST)***:We used GIST cell line,*** hasa heterogenic 57-bp deletion in exon 11 to produce amutated c-KIT,which results in constitutive activationof *** were treated with/without STI571 orstem cell factor(SCF).Transcription and expression ofVEGF were determined by RT-PCR and flow cytometryor Western blotting,*** c-KIT wasestimated by immunoprecipitation *** viabilitywas determined by MTT ***:Activation of c-KIT was inhibited bySTI571 *** was suppressed at both thetranscriptional and translational levels in a temporal anddose-dependent manner by *** upregulatedthe expression of VEGF and it was inhibited by ***571 also reduced the cell viability of the GIST-T1cells,as determined by MTT ***:Activation of c-KIT in the GIST-T1regulated the expression of VEGF and it was inhibited ***571 has antitumor effects on the GIST cellswith respect to not only the inhibition of cell growth,butalso the suppression of VEGF expression.