Determination and pharmacokinetic study of catechin in rat plasma by HPLC

作     者：Li Xie Xin-Nan Li De-Xi Jiang Dan Zhang 

作者机构：West China School of Pharmacy Siehuan University Chengdu 610041 China 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2011年第1卷第4期

页      面：297-301页

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 09[农学] 070302[理学-分析化学] 0710[理学-生物学] 090603[农学-临床兽医学] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 0817[工学-化学工程与技术] 0906[农学-兽医学] 0703[理学-化学] 0702[理学-物理学] 

主　　题：HPLC Determination Pharmac^kinctic Catechin Rat Plasma 

摘      要：A high performance liquid chromatographic method was developed and validated for the quantitative determination of catechin in rat plasma and its pharmacokinetic study after intragastric administration of Catechu and Xiongdanjiangre Wan into SD rats. Plasma samples were prepared by protein precipitation using methanol-5% aqueous zinc sulfate （70：30, v/v） as precipitant. Chromatographic separation was achieved on Hypersil Cl8 column （250 mm~ 4.6 mm, 10 pm） with acetonitrile-water-triethylamine （6：94：0.3, v/v/v, pH 4.0＋0.1, adjusted with phos- phoric acid） as mobile phase, followed by a UV detection at 207 nm. Good linearity was obtained over the range of 0.143-7.15 mg/L of catechin, with correlation coefficient of 0.9992. The method was simple, sensitive, accurate and reproducible and＇ has been successfully applied to the pharmacokinetic study of catechin in rat plasma.