A Perspective on Data Processing in Super-resolution Fluorescence Microscopy Imaging
作者机构：Universitéde LilleLASIR CNRS59655 Villeneuve d’Ascq CedexFrance
出 版 物：《Journal of Analysis and Testing》 (分析检测（英文）)
年 卷 期：2018年第2卷第3期
页 面：193-209页
核心收录：
学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1002[医学-临床医学] 0817[工学-化学工程与技术] 0804[工学-仪器科学与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 100214[医学-肿瘤学] 0702[理学-物理学] 10[医学]
主 题：Super-resolution Fluorescence microscopy Nanoscopy Imaging
摘 要：With super-resolution microscopy,we attempt to visualize(biological)structures and processes at the sub-cellular level(i.e.,nanoscale).To obtain this information,the samples are labeled with fluorophores that have a stochastic on/off switching of their emissions,which help to overcome the optical diffraction limit of around 250 nm,related to the use of optical ***,nowadays,research focuses on the imaging of live cells and thicker *** investigations require a high amount of simultaneously active fluorophores(i.e.,high-density imaging)and are challenging due to the collapse of the single-molecule localization techniques and the increased background in the ***,recent efforts have shifted towards the development of new ways to process the *** publication gives an introduction to wide-field super-resolution fluorescence microscopy,explaining the concepts of the technique,and then gives an overview of the recently developed methods to provide super-resolution images for high-density data of live cells and ways to overcome the issues related to the imaging of these samples.
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