Preparation of ^(99m)Tc-HYNIC-Annexin V

作     者：LAN Xiao-Li ZHANG Yong-Xue 

作者机构：Department of Nuclear Medicine Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 

出 版 物：《Nuclear Science and Techniques》 (核技术（英文）)

年 卷 期：2005年第16卷第5期

页      面：299-303页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 100207[医学-影像医学与核医学] 1006[医学-中西医结合] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 08[工学] 1010[医学-医学技术（可授医学、理学学位）] 100106[医学-放射医学] 100602[医学-中西医结合临床] 10[医学] 

主　　题：HYNIC 放射性医学 同位素 治疗方法 

摘      要：99mTc-HYNIC-Annexin V was prepared for apoptosis radionuclide imaging in vivo. The expression plas-mid of human Annexin V was constructed and expressed in E. coli.. HYNIC was prepared and used as bifunctional chelating agent for 99mTc labeling. The radiochemical purity, radiolabeling yield and stability of 99mTc-HYNIC-Annexin V were studied. The coding sequence of human Annexin V gene was successfully cloned into the expression vector, and highly expressed in E. coli. A protein with a molecular weight of about 36kD could be in-duced and confirmed by Western Blotting and SDS-PAGE. Annexin V could be labeled with 99mTc using HYNIC,and showed high radiochemical purity of more than 90%. The radiolabeling yield could reach more than 90%. The reac-tion condition was moderate. It suggested that 99mTc-HYNIC-Annexin V may be a promising agent for apoptosis im-aging and clinical application.