Effects of Gastrodiae rhizoma on proliferation and differentiation of human embryonic neural stem cells

作     者：Samrat Baral Ramesh Pariyar Chi-Su Yoon Dong-Cheol Kim Jong-Min Yun Seok O Jang Sung Yeon Kim Hyuncheol Oh Youn-Chul Kim Jungwon Seo 

作者机构：From the BK21 Plus Team Institute of Pharmaceutical Research and DevelopmentCollege of PharmacyWonkwang University Professional Graduate School of Oriental MedicineWonkwang University Hanbang Body-Fluid Research CenterWonkwang University Standardized Material Bank for New Botanical DrugsCollege of PharmacyWonkwang University Department of Internal MedicineCollege of Korean MedicineWonkwang University 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2015年第8卷第10期

页      面：776-781页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by Grant of the Traditional Korean Medicine R&D Project,Ministry of Health and Welfare,Republic of Korea(HI13C0516) the National Research Foundation of Korea(NRF)grand funded by the Korea government(MSIP)(No.2008-0062484) 

主　　题：Gastrodiac rhizoma Neural stem cells Neuronal diff 

摘      要：Objective:To investigate the effects of Gastrodiae rhizoma,a dried root of Gastrodia elata Blume,on proliferation and differentiation of human NSCs derived from embryonic stem ***:A 70%ethanol extract of Gastrodiae rhizoma(EEGR) was estimated with4-hydroxybenzyl alcohol as a representative constituent by ***:MTT assay showed that the treatment with EEGR increased the viability of NSCs in growth *** to contro1,EEGR increased the number of dendrites and denritic spines extended from a differentiated *** EEGR decreased the mRNA expression of Nestin,it increased that of Tuj1 and MAP2 in NSCs grown in differentiation *** analysis using confocal microscopy also revealed the increased expression of MAP2 in dendrites of EEGR-treated ***,EEGR decreased mRNA expression of Sox2 in NSCs grown even in growth ***:In conclusion,our study demonstrates for the first time that EEGR induced proliferation and neuronal differentiation of NSCs,suggesting its potential benefits on NSC-based therapies and neuroregeneration in various neurodegenerative diseases and brain Injuries.