Effect of Insulin on Development of ICR Mouse Embryos in Vitro
Effect of Insulin on Development of ICR Mouse Embryos in Vitro作者机构：Department of Gynecology 2nd Affiliated Hospital of Kunming Medical College Kunming 650101 China Department of Gynecology and Obstetrics Renhe Hospital of Three Gorges University Yichang 443001 China
出 版 物：《Journal of Reproduction and Contraception》 (生殖与避孕（英文版）)
年 卷 期：2009年第20卷第4期
页 面：205-210页
学科分类：090504[农学-特种经济动物饲养（含：蚕、蜂等）] 0710[理学-生物学] 07[理学] 0905[农学-畜牧学] 09[农学] 071003[理学-生理学]
主 题：insulin mouse embryo development in vitro
摘 要：Objective To study the effects and the appropriate concentration of insulin on the development of mouse embryos in vitro, and the effects of insulin on the development of different stages of embryos. Methods Mouse embryos were cultured in vitro in the mKSOM media supplemented with insulin at different concentrations and with insulin during different stages of embryos. The blastoeyst rates and the cell numbers were counted. Results Additions of insulin significantly increased the rates of blastocyst and the total cell numbers. The concentrations of 0.005 μg/ml and 0.05 μg/ml insulin caused a significant increase in the total cell numbers compared with the control and experimental groups. Addition of insulin from 2-cell to 4-cell stage or from the 4-cell to morula stage, significantly increased the blastocyst rates compared with control and experi- mental groups. Conclusion Insulin can promote the development of mouse embryos in vitro. The appropriate concentration of insulin added in mKSOM was 0.005 μg/ml and 0.05μg/ml. Exposure of embryos to insulin, beginning at the 2-cell and extending to the 4-cell stage or beginning at the 4-cell and extending to the morula stage, is important for the development of lCR mouse embryos in vitro.
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