High-throughput sequencing reveals similar molecular signatures for class switch recombination junctions for theγandαisotypes

作     者：ussein Issaoui Nour Ghazzaui Alexis Saintamand Yves Denizot François Boyer 

作者机构：UMR CNRS 7276INSERM U1262CBRSLimoges Universityrue Pr.DescottesLimoges 87025France INSERM U1236Rennes 1 UniversityRennesFrance 

出 版 物：《Cellular & Molecular Immunology》 (中国免疫学杂志（英文版）)

年 卷 期：2019年第16卷第1期

页      面：90-92页

核心收录：

学科分类：07[理学] 0701[理学-数学] 070101[理学-基础数学] 

基　　金：This work was supported by ANR(project EpiSwitch-3’RR 2016) N.G.was supported by a grant from Association de Spécialisation et d’Orientation Scientifique(Lebanon)and the municipality of Khiam(Lebanon) H.I.is supported by CORC(FJA/NP 2015-109)and the University of Limoges 

主　　题：functions. process. joining 

摘      要：INTRODUCTION After encountering an antigen,B cells undergo class switch recombination(CSR),which substitutes the Cμgene with Cγ,Cε,or Cαto generate IgG,IgE,and IgA antibodies with the same antigenic specificity but new effector functions.1 The DNAediting enzyme activation-induced deaminase(AID)is essential for CSR by targeting switch(S)regions preceding Cμ(namely,the Sμdonor region)and the Cγ,Cε,and Cαgenes(namely,the Sγ,ε,αacceptor regions).1 Cis-and trans-controlled DNA double strand beaks are generated during this process.2–6 Recruitment of DNA repair factors that facilitate the end-joining process is a crucial step of class switch *** pathways are implicated in this end *** classical non-homogenous end joining(c-NHEJ)pathway ligates DNA ends with no or little *** contrast,the alternative end joining(A-EJ)pathways is used to ligate DNA ends that have microhomology.