Antigen-binding characteristics of AbCD71 and its inhibitory effect on PHA-induced lymphoproliferation

作     者：Ping LEI~2,Yong HE~3,Qing YE~4,Hui-fen ZHU~2,Xiao-mei YUAN~2,Jing LIE~2,Wei XING~2,Sha WU~2,Wei DAI~2,Xin SHEN~2,Guo-bin WANG~(5,6),Guan-xin SHEN~(2,6)Departments of ~2 Immunology and Pharmacology,~3 Nuclear Medicine and ~5 Surgery,Union Hospital,Tongji Medical College,HuazhongUniversity of Science and Technology,Wuhan 430030,China ~4 Department of Pathobiology and Physiology,Medicine and Life ScienceCollege of Jianghan University,Wuhan 430056,China 

作者机构：Department of Immunology and Pharmacology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China Department of Nuclear Medicine Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China Department of Pathobiology and Physiology Medicine and Life Science College of Jianghan University Wuhan China Department of Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2007年第28卷第10期

页      面：1659-1664页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：Project supported by grants from the Hi-Tech Research and Development Program of China(№ 2006AA02Z158) Hubei Key Sci &Tech R&D Programme(№ 2006AA301A05) 

主　　题：AbCD71 chimeric human/murine antibody lymphoproliferation transplantation rejection 

摘      要：Aim:To investigate the anti-lymphoproliferative effect of the prepared recombi-nant chimeric human/murine anti-cluster of differentiation（CD） 71 monoclonalantibody （AbCD71）,which is composed of mouse-derived,antigen-binding vari-able regions and human-derived constant ***:After plasmids con-struction and transfection,the expression of AbCD71 in the transfectoma super-natant was determined by the sandwich *** immunofluorescence as-say was used to measure the antigen-binding characteristic and the percent CD71-expressed peripheral blood mononuclear cells （PBMC）.The antibodies were puri-fied from the ascites via diethylaminoethyl（DEAE）-Sephadex A-50 chromatogra-phy and then identified by *** last,inhibitory effect of AbCD71 onPHA-induced PBMC proliferation was calculated by methyl thiazolyl tetrazolium（MTT） ***:Constant domain of heavy chain（CH） and light chain（CL）ofAbCD71 were in the human Cγfamily and human CKfamily,***71could compete with its original murine mAb to bind with CD71-positive humanleukemia cell line CEM ***71 could inhibit the peripheral blood mono-nuclear cell proliferation induced by phytohemagglutinin（PHA） in vitro in a dose-dependent manner,especially at time-points 0 and 12 h after *** wasno statistical difference when compared with original murine ***:The AbCD71 is a promising *** approach to blocking CD71with the chimeric human/murine mAb provides a novel strategy for prolongingallograft survival.