Gene and protein expression profiling analysis of young spike development in large spike wheat germplasms

作     者：CHEN Dan ZHANG Jin-peng LIU Wei-hua WU Xiao-yang YANG Xin-ming LI Xiu-quan LU Yu-qing LI Li-hui 

作者机构：National Key Facility for Crop Gene Resources and Genetic ImprovementInstitute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081P.R.China Biotechnology and Germplasm Resources InstituteYunnan Academy of Agricultural SciencesKey Laboratory of Southwestern Crop Gene Resources and Germplasm InnovationMinistry of Agriculture/Scientific Observation for Rice Germplasm Resources of YunnanMinistry of Agriculture/Yunnan Provincial Key Laboratory of Agricultural BiotechnologyKunming 650223P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2016年第15卷第4期

页      面：744-754页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by grants from the National Basic Research Program of China(973 Program,2011CB100104) the National High-Tech R&D Program of China(2011AA100101) the National Natural Science Foundation of China(31071416) the National Key Technologies R&D Program of China during the 12th FiveYear Plan period(2013BAD01B02) 

主　　题：wheat high grain number per spike spike development expression profiling 

摘      要：The wheat grain number per spike （GNPS） is a major yield-limiting factor in wheat-breeding programs. Germplasms with a high GNPS are therefore valuable for increasing wheat yield potential. To investigate the molecular characteristics of young spike development in large-spike wheat germplasms with high GNPS, we performed gene and protein expression profiling analysis with three high-GNPS wheat lines （Pubing 3228, Pubing 3504 and 4844-12） and one Iow-GNPS control variety （Fukuho）. The phenotypic data for the spikes in two growth seasons showed that the GNPS of the three large-spike wheat lines were significantly higher than that of the Fukuho control line. The Affymetrix wheat chip and isobaric tags for relative and absolute quantitation-tandam mass spectrometry （iTRAQ-MS/MS） technology were employed for gene and protein expression profiling analyses of young spike development, respectively, at the floret primordia differentiation stage. A total of 598 differentially expressed transcripts （270 up-regulated and 328 down-regulated） and 280 proteins （122 up- regulated and 158 down-regulated） were identified in the three high-GNPS lines compared with the control line. We found that the expression of some floral development-related genes, including Wknoxlb, the AP2 domain protein kinase and the transcription factor HUA2, were up-regulated in the high-GNPS lines. The expression of the SHEPHERD （SHD） gene was up-regulated at both the transcript and protein levels. Overall, these results suggest that multiple regulatory pathways, including the CLAVATA pathway and the meristem-maintaining KNOX protein pathway, take part in the development of the high-GNPS phenotype in our wheat germplasms.