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Screening of Rice Genes Interacting with p5b of Rice BlackStreaked Dwarf Virus

Screening of Rice Genes Interacting with p5b of Rice BlackStreaked Dwarf Virus

作     者:LU Ying YANG Jian ZHANG Heng-mu CHEN Jian-ping 

作者机构:State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control/Key Laboratory of Plant Protection and BiotechnologyMinistry of Agriculture/Zhejiang Provincial Key Laboratory of Plant Virology/Institute of Virology and BiotechnologyZhejiang Academy of Agricultural Sciences College of Plant ProtectionNanjing Agricultural University 

出 版 物:《Rice science》 (水稻科学(英文版))

年 卷 期:2013年第20卷第3期

页      面:185-190页

学科分类:09[农学] 0901[农学-作物学] 

基  金:funded by the National Science and Technology Support Program of China(Grant No.2012BAD19B03) the National High Technology Research and Development Program of China(Grant No.2007AA10Z414) the National Basic Research Program of China(Grant No.2010CB126203) the International Science and Technology Cooperation Project(Grant No.2007DFB30350) the Special Fund for Agro-scientific Research in the Public Interest of China(Grant No.201003031) the Zhejiang Provincial Science and Technology Project,China(Grant No.2010C12027) the Zhejiang Provincial Foundation for Natural Science,China(Grant Nos.Z305165and Y3090657) 

主  题:rice black-streaked dwarf virus interaction protein yeast two-hybrid 

摘      要:Rice black-streaked dwarf virus (RBSDV) is a recognized member of the genus Fijivirus, family Reoviridae. Its genome has ten double-stranded RNA (dsRNA) segments ($1-$10), in which the fifth genome segment ($5) contains two open reading frames (ORFs) with a partially overlapping region. The second ORF of RBSDV S5 encodes a viral nonstructural protein named p5b with unknown function. To reveal the function of p5b, its gene was ligated into the bait plasmid pGBKT7 and an expression library containing rice cDNAs was constructed using plasmid pGADT7 for yeast two-hybrid assay. The bait protein p5b was detected in yeast by western blot, and the result of an auto-activation test showed that p5b could not autonomously activate the expression of reporter genes in yeast. Then the bait protein p5b was used for screening the cDNA expression libraries of rice. Gene fragments of some pivotal enzymes involved in photosynthesis, respiration and other important metabolic processes, were identified to interact with p5b in yeast, suggesting that these interactions may play roles in symptom development in infected plants.

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