Influence of endogenous ciliary neurotrophic factor on neural differentiation of adult rat hippocampal progenitors

作     者：Jun Ding Zhili He Juan Ruan Ying Liu Chengxin Gong Shenggang Sun Honghui Chen 

作者机构：Department of NeurologyUnion HospitalTongji Medical CollegeHuazhong University of Science and Technology Mental Health CenterTongji Medical CollegeHuazhong University of Science and Technology New York State Institute for Basic Research in Developmental DisabilitiesStaten IslandNYUSA 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2013年第8卷第4期

页      面：301-312页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 071006[理学-神经生物学] 

基　　金：supported by the National Natural Science Foundation of China No. 30770754 

主　　题：neural regeneration stem cells spontaneous differentiation neural progenitor cells endogenous neurotrophic factors ciliary neurotrophic factor regeneration grants-supported paper photographs-containing paper neuroregeneration 

摘      要：Ciliary neurotrophic factor is the only known neurotrophic factor that can promote differentiation of hippocampal neural progenitor cells to glial cells and neurons in adult rats. This process is similar to spontaneous differentiation. Therefore, ciliary neurotrophic factor may be involved in spontaneous differentiation of neural stem cells. To verify this hypothesis, the present study isolated neural progenitor cells from adult male rats and cultured them in vitro. Results showed that when neural progenitor cells were cultured in the absence of mitogen fibroblast growth factor-2 or epidermal growth factor, they underwent spontaneous differentiation into neurons and glial cells. Western blot and immunocytochemical staining showed that exogenous ciliary neurotrophic factor strongly induced adult hippocampal progenitor cells to differentiate into neurons and glial cells. Moreover, passage 4 adult hippocampal progenitor cells expressed high levels of endogenous ciliary neurotrophic factor, and a neutralizing antibody against ciliary neurotrophic factor prevented the spontaneous neuronal and glial differentiation of adult hippocampal progenitor cells. These results suggest that the spontaneous differentiation of adult hippocampal progenitor cells is mediated partially by endogenous ciliary neurotrophic factor.