Effect of respiratory syncytial virus on the activity of matrix metalloproteinase in mice

作     者：LI Wen SHEN Hua-hao 

作者机构：Department of Respiratory Medicine Second Affiliated HospitalZhejiang University College of Medicine Institute of RespiratoryDiseases Zhejiang University Hangzhou 310009 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2007年第120卷第1期

页      面：5-11页

核心收录：

学科分类：1002[医学-临床医学] 100202[医学-儿科学] 10[医学] 

主　　题：respiratory syncytial virus matrix metalloproteinase inhibitory agents E-caherin,. proliferating cell nuclear antigen 

摘      要：Background Respiratory syncytial virus （RSV） is a common pathogen in the lower respiratory tract of infants and children. Recent studies have shown that in adults, especially in the eldedy population who have relatively weak immunity, lower respiratory tract infection is not uncommon. RSV was detected in 22% hospitalized patients with acute exacerbation of chronic obstructive pulmonary disease （COPD）, and the detection rate was only next to that of parvovirus and influenza virus respectively. Further studies revealed that lung infection of RSV could lead to inflammatory destruction and structural remodeling. This study was undertaken to examine the effect of infection with RSV on matrix metalloproteinase （MMPs） in mice, and to explore the role of RSV in the pathogenesis of pulmonary diseases. Methods Twenty BALB/c mice were divided randomly into an RSV infection group and a blank control group. The mice in the RSV infection group were given 100 pl liquid containing 10s PFU of RSV by intranasal instillation. Three days after the infection, the bronchoalveolar lavage fluid （BALF） was harvested and RT-PCR and Western blotting were used to detect MMP-9 and the expression of tissue inhibitors of matdx metalloproteinase （TIMP）-I mRNA in lung tissues. Gelatin zymography was employed to detect the activities of MMP-9 and MMP-2 in BALF. Immunohistochemistry was used to determine the expressions of E-cadherin （E-cd） and proliferating call nuclear antigen （PCNA） in the lung tissues. Results In the BALF of the mice infected with RSV, the activities of MMP-9 and MMP-2 were significantly increased （t=-6.08, P〈0.01 and t=5.68, P〈0.01）. The levels of mRNAand proteins of MMP-9 in the lung tissues of the mica infected with RSV were significantly elevated, and the mRNA and protein levels were significantly higher than those of the blank controls. Though the ratio of MMP-9/TIMP-1 mRNA in the lung tissues of the infected mica was not significantly different from that of the normal c