Genetic Diversity of Seed Storage Proteins in Different Ecotype Varieties of japonica Rice and Its Application

作     者：JIN Wei-dong LI Na HONG De-lin 

作者机构：National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China Suzhou Institute of Rural Cadre Suzhou 215011 China 

出 版 物：《Rice science》 (水稻科学（英文版）)

年 卷 期：2006年第13卷第2期

页      面：85-92页

学科分类：09[农学] 0901[农学-作物学] 

基　　金：The projects of Nanjing Agricultural University SRT item(0401A04) 江苏省重点研究项目(96502-0-1) The Cooperative Research between China and Japan(2002-2004) 

主　　题：genetic diversity japonica rice ecotype storage protein electrophoresis 

摘      要：One hundred and fifteen varieties （including cultivars and lines） with different ecotypes in japonica rice （Oryza sativa L.） were analyzed for endosperm storage proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis （SDS-PAGE） to estimate their genetic diversity for the purpose of genetic improving and variety identification. Nineteen types of profile were identified according to 1） presence/absence of 65 kDa bands, 2） staining intensity of 70, 60, 57, 37-39, 22-23, 13 and 10 kDa bands, 3） migration velocity of 35 kDa （α-4） and 4） band number at 57 kDa location. An unweighted-pair group average method with arithmetic mean （UPGMA） dendregram based on the cluster analysis of genetic similarity of the protein bands showed a small genetic variation among the tested materials, with the similarity coefficients varying between 0.75 and 1.00. Three distinct groups were identified from the cluster analysis of the rice varieties studied at the similarity coefficient level of 0.894. The first group included eight varieties with high amylose content, the second group contained fifteen varieties with high protein content, and the third group had the remaining ninety-two varieties, which accounted for 80% of the total materials. Clear relationship between ecotypes distinguished by maturity and groups revealed by cluster analysis was not found in this study. Only the group of high amylose linked with medium-maturity medium japonica ecotype. The bands of 70 kDa and 65 kDa can be used as protein markers to identify F1 seed purity of japonica hybrid rice Liuyanyou 422.