Nogo-A expression dynamically varies after spinal cord injury

作     者：Jian-wei Wang Jun-feng Yang Yong Ma Zhen Hua Yang Guo Xiao-lin Gu Ya-feng Zhang 

作者机构：Wuxi Hospital of Traditional Chinese Medicine Institute of Orthopedics and Traumatology of Nanjing University of Chinese Medicine Nanjing University of Chinese Medicine 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2015年第10卷第2期

页      面：225-229页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：financially supported by a grant from the Natural Science Foundation of Jiangsu Province,No.BK2011180 Ordinary University Graduate Student Scientific Research Innovation Projects of Jiangsu Province,No.CXZZ13-0614,CXZZ12-0609 

主　　题：nerve regeneration spinal cord injury contusion Nogo-A axon growth immunohisto-chemistry fluorescent quantitative PCR neural regeneration 

摘      要：The mechanism involved in neural regeneration after spinal cord injury is unclear. The my-elin-derived protein Nogo-A, which is speciifc to the central nervous system, has been identiifed to negatively affect the cytoskeleton and growth program of axotomized neurons. Studies have shown that Nogo-A exerts immediate and chronic inhibitory effects on neurite *** vivo, inhibitors of Nogo-A have been shown to lead to a marked enhancement of regenerative axon extension. We established a spinal cord injury model in rats using a free-falling weight drop device to subsequently investigate Nogo-A expression. Nogo-A mRNA and protein expression and immunoreactivity were detected in spinal cord tissue using real-time quantitative PCR, immu-nohistochemistry and western blot analysis. At 24 hours after spinal cord injury, Nogo-A protein and mRNA expression was low in the injured group compared with control and sham-operated groups. The levels then continued to drop further and were at their lowest at 3 days, rapidly rose to a peak after 7 days, and then gradually declined again after 14 days. These changes were observed at both the mRNA and protein level. The transient decrease observed early after injury followed by high levels for a few days indicates Nogo-A expression is time dependent. This may contribute to the lack of regeneration in the central nervous system after spinal cord injury. The dynamic varia-tion of Nogo-A should be taken into account in the treatment of spinal cord injury.