Induction of Differentiation and Apoptosis in Three Human Leukemia Cell Lines by a New Compound from Dendrostellera lessertii

作     者：Razieh YAZDANPARAST Majid MAHDAVI Mohammad Amin MOOSAVI 

作者机构：Institute of Biochemistry and BiophysicsUniversity of TehranTehranlran 

出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))

年 卷 期：2006年第38卷第7期

页      面：477-483页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：apoptosis Dendrostellera lessertii differentiation macrophage megakaryocyte 

摘      要：It has previously been shown that Dendrostellera lessertii（Thymelaeaceae）has stronganticancer *** this study,the antileukemic activity of another new compound from the same plantextract is ***（NB4 and HL-60）and erythroleukemia（K562）cells were cultured in thepresence of various concentrations of the new compound（0.5-3.0 μtg/ml）for *** cell numbers werethen determined by trypan blue exclusion *** new compound inhibited growth and proliferation ofNB4,HL-60 and K562 with IC50values of 1.5,2.0 and 2.5μg/ml,*** also found that the newcompound inhibited cell proliferation in a dose-and time-dependent *** low concentrations and after48h of treatment,approximately 50%-70% of NB4 and HL-60 cells were differentiated to monocyte/macrophage lineage and approximately 30%-40% of the treated K562 cells were differentiated in the mega-karyocytic lineage,as evidenced by morphological changes and nitro blue tetrazolium reduction *** of Hoechst 33258 staining also indicated that the new compound induced NB4 and HL-60 cellapoptosis at their respective IC50values after 72h of *** on the present data,the new com-pound seems a good candidate for further evaluation as an effective chemotherapeutic agent acting throughinduction of differentiation and apoptosis.