Reduced function and disassembled microtubules of cultured cardiomyocytes in spaceflight

作     者：YANG Fen LI YingHui DING Bai NIE JieLin WANG HongHui ZHANG XiaoYou WANG ChunYan LING ShuKuan NI ChengZhi DAI ZhongQuan TAN YingJun WAN YuMin 

作者机构：Laboratory of Space Cell and Molecular Biology China Astronaut Research and Training Center Beijing 100094 China 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2008年第53卷第8期

页      面：1185-1192页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：China Manned Space Engineering Project, National Basic Research Program of China (Grant No. 2006CB705F04) National High Technology Research and Development Program of China (Grant No. 2002AA743051) National Natural Science Foundation of China (Grant No. 30370365) Advanced Space Med-ico-Engineering Research Project of China (Grant No. SJ200508) 

主　　题：细胞骨架 尿钠排泄 肽 航天飞行 心脏 

摘      要：Lack of gravity during spaceflight has profound effects on cardiovascular system, but little is known about how the cardiomyocytes respond to microgravity. In the present study, the effects of spaceflight on the structure and function of cultured cardiomyocytes were reported. The primary cultures of neo- natal rat cardiomyocytes were carried on Shenzhou-6 spacecraft and activated at 4 h in orbit. 8 samples were fixed respectively at 4, 48 and 96 h after launching for immunofluorescence of cytoskeleton, and 2 samples remained unfixed to analyze contractile and secretory functions of the cultures. Ground sam- ples were treated in our laboratory in parallel. After 115 h spaceflight, video recordings displayed that the number of spontaneous beating sites in flown samples decreased significantly, and the cells in the beating aggregate contracted in fast frequency without synchrony. Radioimmunoassay of the medium showed that the atrial natriuretic peptide secreted from flown cells reduced by 59.6%. Confocal images demonstrated the time-dependant disassembly of mirotubules versus unchanged distribution and or- ganization of microfilaments. In conclusion, above results indicate reduced function and disorganized cytoskeleton of cardiomyocytes in spaceflight, which might provide some cellular basis for further investigations to probe into the mechanisms underlying space cardiovascular dysfunction.