Using yeast two-hybrid system to detect interactions of ATP synthase subunits from Spinacia oleracea

作     者：石晓冰 魏家绵 沈允钢 

作者机构：Shanghai Institute of Plant Physiology Chinese Academy of Sciences Shanghai 200032 China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2000年第43卷第2期

页      面：169-175页

学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：the State Key Basic Research and Development Plan the National Natural Science Foundation of China(39730040) 

主　　题：ATP synthase subunit interaction yeast two-hybrid system. 

摘      要：Subunit interactions among the chloroplast ATP synthase subunits were studied using the yeast two-hybrid system. Various pairwise combinations of genes encoding a, p, y, 8 and e subunits of Spinach ATP synthase fused to the binding domain or activation domain of GAL4 DNA were introduced into yeast and then expression of a reporter gene encoding p-galactosidase was detected. Of all the combinations, that of y and e subunit genes showed the highest level of reporter gene expression, while those of a and p, a and e, p and e and p and 8 induced stable and significant reporter gene expression. The combination of 8 and e as well as that of 8 and y induced weak and unstable reporter gene expression. However, combinations of a and y, p and y and a and 8 did not induce reporter gene expression. These results suggested that specific and strong interactions between y and e, a and p, a and e, p and e and p and 8 subunits, and weak and transient interactions between 8 and e and 8 and y subunits occurred in the