Clinicopathological Features of Non-familial Colorectal Cancer with High-frequency Microsatellite Instability

作     者：Peng Jin Xiao-ming Meng Jian-qiu Sheng Zi-tao Wu Lei Fu He-juan An Ying Han Shi-rong Li 

作者机构：Department of Gastroenterology The Military General Hospital of Beijing Beijing 100700 China 

出 版 物：《Chinese Medical Sciences Journal》 (中国医学科学杂志（英文版）)

年 卷 期：2010年第25卷第4期

页      面：228-232页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1002[医学-临床医学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 071005[理学-微生物学] 090102[农学-作物遗传育种] 10[医学] 

基　　金：Supported by National Natural Science Foundation of China (30940086) 

主　　题：colorectal cancer microsatellite instability phenotype clinical pathology 

摘      要：Objective To explore the clinicopathological features of non-familial colorectal cancer with high-frequency microsatellite instability (MSI-H). Methods One hundred and fifty patients with colorectal cancer who had no family history were enrolled in this study from June 2006 to June 2008. Five standard microsatellite loci including BAT25, BAT26, D2S123, D5S346, and D17S250 were amplified with immunofluorescent polymerase chain reaction. The patient information including age, sex, and tumor location was recorded. Pathological features including differentiation, mucinous differentiation, histological heterogeneity, and Crohn s-like reaction were observed under light microscope. The presence of tumor-infiltrating lymphocytes (TLs, CD4+ and CD8+) was detected by means of immunohistochemistry. A regression equation was obtained by stepwise logistic regression analysis to evaluate the relationship between MSI-H phenotype in colorectal cancer ands pathological features. Results MSI-H phenotype occurred in 13.33% of the 150 patients with non-familial colorectal cancer. Poor differentiation, histological heterogeneity, Crohn s-like reaction, and presence of TLs were found to be independent factors to identify MSI-H non-familial colorectal cancer. Logistic regression equation showed an overall sensitivity of 70.0%, specificity of 99.2%, and accuracy of 95.3% in identifying MSI-H non-familial colorectal cancer. Conclusion MSI-H non-familial colorectal cancer manifests specific pathological features, which may be relied upon for effective identification of that disease.