Gradual Clamping Reduced Ischemia-Reperfusion Injury in an Isolated Rat Heart Model
Gradual Clamping Reduced Ischemia-Reperfusion Injury in an Isolated Rat Heart Model作者机构：Department of Anesthesia The Sixth Affiliated Hospital Sun Yat-sen University Guangzhou China Department of Anesthesia Tianjin Fifth Central Hospital Tianjin China
出 版 物：《World Journal of Cardiovascular Surgery》 (心血管外科国际期刊（英文）)
年 卷 期：2016年第6卷第6期
页 面：79-86页
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学]
主 题：Gradual Clamping Ischemia-Reperfusion Injury Gradual Adaptation Rat Heart Model
摘 要：Objectives: We hypothesized that the organisms and their organs or tissues could adapt themselves to the gradual changes of environment for surviving or reducing damage. This study explored whether gradual clamping (GC) could reduce myocardial ischemia-reperfusion (IR) injury in rat heart. Methods: Twelve rats were randomized to IR group and GC group, then the hearts were isolated and perfused with Langendorff apparatus. Before cardioplegia, the perfusion was stopped abruptly in IR group while slowly with 5-minute in GC group. The hearts were subjected to 30-minute ischemia and 60-minute reperfusion. The left ventricular develop pressure (LVDP) and systolic pressure (LVSP), the maximal rate of the increase and decrease of left ventricular pressure (+dp/dtmax, ﹣dp/dtmax) were measured by polygraph system at different time points. The recovery of the variables was expressed as the ratio of these values at individual time point after reperfusion to the baseline respectively. Results: The recovery of LVDP after reperfusion was better than that in IR group (P = 0.034). No significant difference in the recovery of LVSP, +dp/dtmax and ﹣dp/dtmax between groups was observed. Conclusions: Gradual clamping could improve the recovery of LVDP after IR, suggesting that gradual clamping could reduce myocardial IR injury.
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